The commercials will run throughout the entire 2017 AFL season on Channel Seven's Saturday AFL broadcast.
Swisse, which was bought by Hong Kong-listed Biostime for $1.67bn in 2015, has been the AFL's vitamin partner since 2010. The firm also announced that Daisy Pearce, Patrick Dangerfield, Shaun Burgoyne and Josh Kennedy will be this season's brand ambassadors, and all feature in the latest campaign.
Swisse general manager of marketing Josh Wittner said: "AFL is a force that brings together so many passionate people who are dedicated to the game - from players, their families, to supporters, coaches and communities, it involves so many.
"We really wanted this year's campaign to highlight and celebrate the support and hard work that goes into the sport, both on and off the field. From the hours of preparation in between games to the dark morning training sessions, there is a relentless passion that brings communities together."